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Bioanalytical method development

Aim:

To develop a selective method with suitable sensitive to quantify the test item and/or its metabolite(s) in biological matrices.

Procedure:

  • Optimization of method parameters
  • Development of suitable sample clean-up method
  • Development of suitable chromatography for LC/MS/MS
  • Investigation of linearity, accuracy, lower limit of quantification (LLOQ), matrix effect and short term stability in the matrix

Evaluation:

Method description includes all materials, instruments and disposables used, as well as preparation of solutions and samples, the sample processing, chromatographic conditions, detection parameters, data evaluation and representative chromatograms.

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