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Colony forming assay in V79 cells

Aim:

Cytotoxicity screening of biomaterials or medical devices based on colony formation of V79 cells.

Test system:

In vitro assay. The V79 lines of Chinese hamster cells has a stable growth curve and as such a high rate of colony formation therefore they are suitable for screening cytotoxicity test.

Design:

Cells are treated with the test item. After incubation, when the colonies are formed and observed, the cells are stained.

Outcome:

The cell survive is determined as the plating efficiencies (PE) ratio.

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