To investigate the rate and extent of the excretion of total radioactivity in bile.
In life phase:
Bile duct cannulated rats are administered with the radio-labeled test item (typical labels: 3H or 14C).
Bile samples are collected in fractions for a defined period post-dose.
The weight of the collected bile samples are measured and suitable aliquot(s) are subjected to liquid scintillation spectroscopy to quantify the total radioactivity in the samples.
For 3H: checking of the potential loss of label due to tritium-proton exchange.
Evaluation for the total radioactivity:
On request, selected bile samples can be stored or transported to dedicated place for further metabolic study.