Auri Knowledge
Start a conversation
Request Proposal
Auri Knowledge

In vitro inter-species comparison of metabolism

Aim:

To compare the metabolic behavior of the test item in different species in in vitro tests.

Test system:

In vitro tests with liver microsomes, hepatocytes, or enzyme preparations.

Procedure:

The test is generally performed in a system containing liver microsomes, specific systems or hepatocytes. (Tests applying special systems, e.g. supersomes or specific isoenzymes are also possible). Only phase I metabolites will be detected in the samples of a microsomal model, while both phase I and phase II metabolites can be studied in samples originating from a system using hepatocytes. Both radio-labeled and non-labeled material can be applied for in vitro metabolic profiling tests. Metabolites of samples of non-labeled test substance are analyzed by LC/MS/MS while those produced from radio-labeled material by HPLC with on-line radioactivity detection (HPLC/RAD). As for the quantitative relations (metabolite mass balance: abundance of the different metabolites) LC/MS/MS provides less accurate, while HPLC/RAD more accurate data (on molar basis). However, LC/MS/MS serves additional information concerning the molecular weight and structure of the metabolites produced.

Outcome:

In vitro tests are most suitable for the comparison of the species since microsomal preparations and hepatocytes of many species are available. Eventual species specific metabolites can also be detected.

Start a conversation
Request Proposal
Auri Knowledge
Auri Knowledge