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July 09, 2016

Mammalian chromosome aberration test

Aim:

The purpose of the in vitro chromosome aberration test is to identify agents that cause structural chromosome aberrations (chromosome or chromatid) in cultured mammalian cells.

Test system:

In vitro test with cultures of cell lines or primary cell cultures.

Design:

At least three test item concentrations from duplicate cultures should be evaluated, negative and positive control should be included.

The cell cultures are exposed to the test item/controls both with and without an exogenous source of metabolic activation.

Additionally, the cell cultures are treated with a metaphase-arresting substance (e.g. colchicine).

After the exposure period, the cells are stained and metaphase cells are analysed microscopically for the presence of chromosome aberrations.

Outcome:

  • Cytotoxicity with and without metabolic activation
  • Percentage of cells with structural chromosome aberration(s), chromatid- and chromosome-type aberrations, gaps

A test item is regarded as non-mutagenic in this assay if there is no:

  • dose-related increase of findings
  •  measured points are statistically significant higher than the concurrent negative control
  • positive results are reproducible and outside the range of the historical negative control data

The biological relevance of the results should be considered.

Contact: Aurigon

Phone: +49 (0)89 780 7200 10
E-Mail: info(at)aurigon.de

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