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T-cell dependent antibody response (TDAR)

Aim:

The aim of this assay is to assess the ability of a test system to mount an antibody response (IgM and/or IgG) to immunization with e.g. KLH (Keyhole limpet hemocyanin) in the presence or absence of co-administered test or reference item. Antigens – such as KLH - are referred to as T-dependent when B lymphocytes require the support of T-cells to elicit an optimal antigen-specific antibody response. TDAR is a primary first line functional assessment of immune system used to monitor the effects of drugs on the immune system.

Species:

Rat or mouse.

Design:

Five groups with a predefined number of animals per sex and group are used:

  • 1. Only KLH (to calculate the magnitude of anti-KLH response in absence of test article
  • 2. Immunosupressant (positive) control, e.g. cyclosporine
  • 3. - 5. Low, mid and high dose (test item) groups

Route and scheme of administration should be the same as in the toxicity study.

All groups are pre-immunized with KLH via tail vein.

Outcome:

KLH-specific IgG and IgM antibody will be detected in serum samples by ELISA and the possible effect of test item treatment upon the immunoresponse to KLH immunization will be evaluated.

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